Correlation studies of rCBV with VEGF protein expression and MVD in gliomas
- VernacularTitle:神经胶质瘤相对性脑血流容积与血管内皮生长因子蛋白表达及微血管密度的相关性研究
- Author:
Benqiang YANG
;
Jianming TIAN
;
Lijuan ZHOU
;
Wenyuan LIU
;
Changqun GUAN
;
- Publication Type:Journal Article
- Keywords:
Magnetic resonance imaging;
Glioma;
Relative cerebral blood volume;
Vascular endothelial growth factor;
Microcirculation;
Neovascularization, pathologic
- From:
Chinese Journal of Radiology
2001;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the correlation of rCBV with vascular endothelial growth factor (VEGF) protein expression and microvessel density (MVD) in gliomas Methods MR examinations were performed preoperatively in 46 patients with suspected supratentorial gliomas All the 46 cases were proved by operation and pathology Immunohistochemical stain methods were used to demonstrate the situation of VEGF protein expression and quantitatively measure the MVD in gliomas The procedures of MR examinations included plain MR scan, PWI and routine contrast enhanced MR scan The pulse sequence of PWI was single shot GRE EPI T 2WI The CBV maps were calculated from the original data of perfusion images and the maximum rCBV of gliomas was acquired from CBV maps through measurement on the region of interest (ROI) According to the situation of VEGF protein expression, all the 46 cases were divided into two groupsincluding positive VEGF protein expression group and negative VEGF protein expression group Mann Whitney U test was used for comparing the difference between the two groups Spearman′s rho correlation analysis was used for observing the correlation between maximum rCBV and MVD in gliomas Results Of the 46 cases; 12 cases were astrocytomas, 3 were oligodendrogliomas, 1 was mixed glioma, 14 were anaplastic astrocytomas, and 16 were glioblastomas multiforme The maximum rCBV value in VEGF(-) group ( n =14) and VEGF(+) group ( n =32) ranged from 0 33～6 63 and 1 03～10 68, with median of 3 08 and 5 95, respectively The difference in maximum rCBV between the two groups was statistically significant ( Mann Whitney U test | z| =2 638, P
