The change in metabolism of fatty acids in breast cancer cells and its significance
- VernacularTitle:乳腺癌中脂肪酸代谢的异常变化及其意义
- Author:
Wenwei CAI
;
Tianfang HUA
;
Jun CAO
;
Qing GU
;
Dun SHI
;
- Publication Type:Journal Article
- Keywords:
Breast cancer;
Metabolism of fatty acid;
Fatty acid synthase;
G 2/M;
Cerulenin
- From:
Parenteral & Enteral Nutrition
1997;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objectives:To observe the changes in metabolism of fatty acids in breast cancer cells and the effects of inhibiting fatty acid synthase(FAS) on the growth of breast cancer cells. Methods:By RT PCR,the expression of FAS mRNA in breast cancer cell line was examined.The growth inhibition of breast cancer cells by specific FAS inhibitor, cerulenin, was determined by MTT assay.Flow cytometric analysis was used to study the changes of cell cycle. Results:FAS mRNA expression in MCF 7 cells was high.We found that cerulenin caused dose and time dependent inhibition of growth of MCF 7 cells.The growth inhibition of MCF 7 after 48 hours exposure of cerulenin at 2.5,5,10 and 20 mg/L was ( 43.47 ?4.58)%,(62.92?2.68)%,(81.93?0.91)% and (67.7?12.27)%( P
