Developmental competence of pig oocytes maturation in different culture mediums
- VernacularTitle:猪卵母细胞在不同培养液中成熟后的发育潜力
- Author:
Huahu YE
;
Hong WEI
;
Junying CHEN
;
- Publication Type:Journal Article
- Keywords:
oocytes;
in vitro maturation;
in vitro fertilization;
dbcAMP;
pig
- From:Journal of Third Military Medical University
1988;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To optimize systems of pig embryo in vitro production. Methods Pig oocytes were matured and fertilized in vitro in different culture mediums. Maturation rate and cleavage rate were analyzed. Results Gonadotropin induced maturation of pig oocytes in vitro , and improved the frequence of oocytes developed into M Ⅱ. The highest maturation and cleavage rates, present in medium with 15 IU/ml FSH+30 IU/ml LH, were 62% and 15% respectively. There was no difference in maturation rates in M199 and NCSU 23(48.2% vs 56.9%). However, difference in cleavage rate was observed in these two mediums(15.7% vs 36%, P
