Selection of specific ouabain conjugated peptides and identification of its binding activity
- VernacularTitle:特异性哇巴因结合肽的筛选及其结合活性的测定
- Author:
Mingjuan ZHANG
;
Zhuoren LU
;
- Publication Type:Journal Article
- Keywords:
peptide library;
ouabain conjungated peptide;
binding
- From:
Chinese Pharmacological Bulletin
1987;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To select specific ouabain conjugated peptides (OCP) to decline ouabain level. METHODS Biopanning phage displayed 12 peptide library for OCP. The sequences of each selected peptide was determined and analyzed. The binding activity of synthetical OCP was identified by radioligand binding assay (RRA). RESULTS Three kinds of peptides were found out. Peptide A (12 peptide) was occupied in 0 67(8/12). The analysis of protein showed that there was no homogeneous protein in Genebank. The results of RRA showed that there were binding activity between synthetical OCP and 3H ouabain. Dissociation constant(K d) was 1 087 nmol?L -1 . Receptor density was 120 pmol?g -1 protein. IC 50 =1 46?10 -4 mol?L -1 . CONCLUSION It is important to obtain distinctive OCP, by which not only the protein sequence was analyzed, but also a valuable experimental data in detection of ouabain and therapy in hypertension also was offered.
