Effect of phosphonoformic acid on vascular smooth muscle cell calcification induced by elevated phosphate
- VernacularTitle:膦甲酸钠干预高磷诱导的血管平滑肌细胞钙化的研究
- Author:
Weiping LU
;
Xiaoyun WANG
;
Xiufen ZHAO
;
- Publication Type:Journal Article
- Keywords:
phosphate;
calcification;
smooth muscle cell;
osteocalcin;
phosphonoformic acid
- From:
Chinese Pharmacological Bulletin
1987;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To observe the effect of phosphonoformic acid (PFA) in different concentration on vascular calcification induced by elevated phosphate. METHODS The cell culture of bovine aortic smooth muscle were performed. Calcium deposition in different phosphate and PFA concentrations were determined by O cresolphthalein complexone method, and osteocalcin expression by radioimmunity and RT PCR. RESULTS Compared to Pi 1 5 mmol?L -1 group,bovine smooth muscle cells (BSMC) cultured in medium containing Pi 2 0 mmol?L -1 phosphate level increased calcium deposition 〔On day 6, (77 187?11 692) mg?g -1 Pro versus (25 768?1 750) mg?g -1 Pro, P
