STUDY ON THE INHIBITED PROLIFERATION AND G_2/M ARREST OF HUMAN GASTRIC CARCINOMA CELLS INDUCED BY GENISTEIN IN VITRO
- VernacularTitle:金雀异黄素抑制人胃癌细胞增殖与G_2/M期阻滞作用的体外研究
- Author:
Hongbin CUI
;
Danfeng SONG
;
Xiaolin NA
;
Xiaoxing CHI
;
Binfeng JIN
;
- Publication Type:Journal Article
- Keywords:
genistein;
gastric carcinoma cells;
growth inhibition;
G 2/M arrest
- From:
Acta Nutrimenta Sinica
1956;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effects of genistein on the proliferation and cell cycle progression of human gastric carcinoma cells. Methods: 3H TdR incorporation test was used to investigate the cell proliferation. Flow cytometry was used to analyze the cell cycle arrest. Immunocytochemistry technique and Western blotting were used to observe the cyclin B and P21 waf1/cip1 protein expression. Results: Genistein inhibited the proliferation of tumor cells significantly, arrested cell cycle progression at G 2/M phase, and enhanced cyclin B and P21 waf1/cip1 protein expression in dose dependent manner. Conclusion:Proliferatory inhibition and G 2/M arrest of human gastric carcinoma cells after treated with genistein may be due to increased stability of cyclin B protein and the expression of P21 waf1/cip1 .
