Inhibitory effect of?-ray irradiation on rat vascular smooth muscle cells
- VernacularTitle:?射线对大鼠血管平滑肌细胞增殖和周期影响
- Author:
Yong-Zhi ZHUANG
;
Jun-Jie WANG
;
Xin-Chen SUN
;
Ting-Zhen JIA
;
- Publication Type:Journal Article
- Keywords:
Vascular smooth muscle cells;
?-ray;
Cell cycle;
Gene expression;
Rat
- From:
Chinese Journal of Radiation Oncology
1992;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism of the inhibitory effect of ?-ray irradiation on rat vascular smooth muscle cells(VSMC). Methods The ef fect of ?-ray irradiation on proliferation of VSMC was observed by 3?H-TdR incor poration. After ?-ray irradiation, the VSMC cell cycle change was detected b y flo w cytometry. The expression of p53, cyclin D and PCNA was investigated by Wester n Blot. Results The inhibitory effect of ?-ray irradiation on VSMC proliferati on was dose-dependent. After ?-ray irradiation, VSMC was arrested in G 1 st age, w ith the expression of p53 increased but the expression of cyclin D and PCNA decr eased. Conclusions ?-ray irradiation can inhibit the proliferation of VSMC. T he main mechanism is probably due to the induction of cell cycle arrest and inhi bition of the VSMC mitosis ,during which process, p53,cyclin D and PCNA all pla y an important role .
