Protective effect of chloroquine on endotoxemia mice and its influence on cytokines
- VernacularTitle:氯喹对内毒素血症小鼠的保护作用以及对细胞因子的影响
- Author:
Jiang ZHENG
;
Hong ZHOU
;
Yongling LU
;
Guangxia XIAO
;
- Publication Type:Journal Article
- Keywords:
chloroquine;
endotoxemia;
mortality;
cytokine
- From:Journal of Third Military Medical University
1988;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect of chloroquine on endotoxemia mice and its inhibition on the release of cytokines induced by LPS. Methods A total of 40 mice of Kunming species were randomly divided into four groups: LPS group received LPS at 10 mg/kg, chloroquine group received chloroquine at 20 mg/kg, LPS plus chloroquine group received chloroquine at 20 mg/kg first, then LPS at 10 mg/kg and control group received only 0.9%sodium chloride at 200 ?l/20 g. The mortality was observed within seven days after injection via caudal vein. ANA 1 cell lines were cultivated in vitro . After chloroquine was first added into the cells for 3 hours, the releases of TNF ? and IL 6 in the supernatants induced by different concentrations of LPS were measured. Results Chloroquine could decrease the death of mice due to endotoxin. Mortality dropped from 100% to 50% ( P
