An analysis of the organic extracts from source water and drinking water in city C and the effects on DNA damage in primary hepatocytes in rats
- VernacularTitle:C市枯水期饮用水中有机污染物分析及对肝细胞DNA损伤的研究
- Author:
Zhiqun QIU
;
Weiqun SHU
;
Huaijun TIAN
;
- Publication Type:Journal Article
- Keywords:
organic extract;
drinking water;
single cell gel electrophoresis;
primary rat hepatocyte;
DNA damage
- From:Journal of Third Military Medical University
1988;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of the polluted organic substances in urban water environment and genetoxicity of the organic pollutants in drinking water on DNA damage in primary cultured hepatocytes in rats. Methods Qualitative analysis of the organic extracts from source water and drinking water in five waterworks in city C with GC/MS was conducted and the effects of organic extracts on DNA damage in primary cultured hepatocytes in rats were tested with single cell gel electrophoresis assay. Comet length means and percentage of tail cell were observed. Results A total of 98 organic pollutants were detected in all samples including 60 in source water and 58 in drinking water. The pollutants were mainly esters, ketons, phenols, benzenes and the derivatives. Comet length means was found to be significantly higher ( P
