Establishment of orthotopic implantation model of human CHG-5 glioma cell line in nude mice and analysis of its biological features
- VernacularTitle:人CHG-5胶质瘤细胞裸鼠原位移植模型的建立及其生物学特性分析
- Author:
Chengping XU
;
Xiuwu BIAN
;
- Publication Type:Journal Article
- Keywords:
glioma;
animal model;
orthotopic implantation;
nude mice
- From:Journal of Third Military Medical University
1983;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an orthotopic implantation model of human glioma in nude mice and investigate its biological features. Methods The human CHG 5 glioma cells were inoculated into brains of nude mice. The animals were sacrificed at day 8, 14, 19, 24 and 30 after inoculation. The tumors were examined with light microscope, electron microscope, karyotype analysis and immunohistochemical stain for glial fibrillary acidic protein. Results Gliomas were formed in 100% in nude mice, and the growth of tumors was stable. The tumors showed the morphological features of human glioma with the immunophenotype. Conclusion The glioma model in nude mice is a reliable animal model for the study of the tumorigenesis and biological characteristics and therapy. The 14th day after inoculation might be suitable for experimental study.
