The loss of heterozygosity on chromosome 3 of plasma free DNA of small cell lung cancer
- VernacularTitle:小细胞肺癌血浆中游离性DNA的3p位点杂合性缺失
- Author:
Bin YI
;
Heping YANG
;
Zhihua RUAN
;
- Publication Type:Journal Article
- Keywords:
lung neoplasm;
LOH;
plasma DNA
- From:Journal of Third Military Medical University
1983;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the diagnostic and monitoring values of the loss of heteroxygosity(LOH) of 3p loci in free plasma DNA of small cell lung cancer. Methods PCR silver staining was used for the detection of LOH of three microsatellite sites in the 3p loci of plasma free DNA and tissue of SCLC. Results In 33 cases of tissue, the incidence rate of LOH was 54.5%(18/33), but the positive rate of LOH in plasma free DNA was 42.4%(14/33). The correlation between them was 78.8%(14/18). Conclusion The plasma free DNA in patients with lung cancer is primarily originated from the tumor tissue. LOH of plasma free DNA may be valuable molecular markers in the diagnosis of SCLC.
