Mutatitions of X region nucleotide sequence of hepatitis B virus DNA in the patients with chronic hepatitis B
- VernacularTitle:乙型肝炎病毒X区核苷酸序列变异的检测
- Author:
Guoping ZHOU
;
Jingyu BAI
;
Xiating LI
;
Al ET
;
- Publication Type:Journal Article
- Keywords:
Hepatitis B virus;
Nucleotides;
Sequence analysis
- From:
Chinese Journal of Infectious Diseases
1999;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mutations of HBV X region nucleotide sequence in the patients with chronic hepatitis B. Methods X region fragments of HBV DNA from 24 patients were amplified by polymerase chain reaction and sequenced. Results There were 2 15 point mutations in X region from the 24 hepatitis patients studied. The A to T mutation at nt1762 and G to A mutation at nt 1764 were found in 11 cases. The combined mutation at nt1636-1741 were found in 8 cases.Conclusions The results indicated the combined mutations of nucleotide(nt) 1762 and 1764 was associated with HBeAg(-) in the patients with chronic hepatitis.
