Effect of phospholipase D on cytoskeieton of glomerular mesangial cells cultured in high glocose
- VernacularTitle:磷脂酶D对高糖培养的肾小球系膜细胞骨架的影响
- Author:
Song-Min HUANG
;
Hai-Yan ZHANG
;
Yong XU
;
Zhao-Hui SHA
;
Xiao-Hong TANG
;
- Publication Type:Journal Article
- Keywords:
Phospholipase D;
Cytoskeleton;
Protein kinase C;
Mesangial cell
- From:
Chinese Journal of Nephrology
1997;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the changes of phosphatidylcholine-specific phospholipase D (PC-PLD) enzyme activity of glomerular mesangial cells (GMCs) in high glucose and the effect of PLD on cytoskeleton. Methods Rats GMCs were cultured in high glucose(30 mmol/L) for 48 hours. PLD enzyme activity was measured by enzyme coupled colorimetry. PKC activity was measured by substrate phosphorylation, and fluorescence intensity of F-actin was observed by FITC-labeled antibody and laser scan cofocal microscopy (LSCM) . Results In high glucose, PLD and PKC activities were obviously elevated, but fluorescence intensity of F-actin was decreased and its cytoskeletal pattern was disassembly. After treatment with inhibtor of PLD, PKC activity was significantly decreased, and intensity and organization of F-actin were recovered. Conclusion Elevation of PLD activity induced by high glucose may influence the cytoskeleton and contraction of CMC through PKC pathway.
