A TLC-HPLC method to analyze taxol in leaf of Torreya Arn.plant
- VernacularTitle:TLC-HPLC法分析榧属植物叶中的紫杉醇
- Author:
Hong ZHANG
;
Zhende CHEN
;
Zhengliang LIU
;
- Publication Type:Journal Article
- Keywords:
thin layer chromatography;
high performance liquid chromatography;
taxol;
Torreya Arn.
- From:
Academic Journal of Second Military Medical University
1982;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
A TLC condition has been developed to purify the extraction from the leaf of Torreya Arn. plant,then an optimized HPLC method was used to determine the amount of taxol.HPLC chromatographic conditions were:colum:Shim Pack CLC ODS(150 mm?6.0 mm,5 ?m);mobile phase:methanol water(66∶34);flow rate: 0.5 ml/min;wavelength of the detector: 227 nm;colum temperature: 35℃.Using this method,we have determined the trace level of taxol in the leaf of Torreya Arn .plant successfully.There exists better linear relationship between peak height( Y ) and the concentration of detected sample( X ) within 100 500 ?g/ml.The average recovery rate is 85.7%( n =12);RSD is 7.8%.
