Construction of antisense VEGF165 eukaryotic expression plasmid
- VernacularTitle:反义VEGF165真核载体的构建
- Author:
Jianhua WANG
;
Yuesong WU
;
Shengdong HUANG
;
Chunpeng JIANG
;
- Publication Type:Journal Article
- Keywords:
vascular endothelial growth factor;
antisense gene
- From:
Academic Journal of Second Military Medical University
1982;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Vascular endothelial growth factor (VEGF)165 cDNA were inserted into the expression plasmid pcDNA3.1 site in an antisense orientation to produce antise VEGF165 expression vector.Then electrophoresis and sequencing were carried out,the antisense vector were verified and could be used for related study of antisense gene expression.