Expression of Janus kinase protein in cocultured PASMC in hypoxia
- VernacularTitle:低氧时复合培养的肺动脉平滑肌细胞Janus激酶蛋白表达的状况
- Author:
Deying CHEN
;
Wenqin CAI
;
Guansong WANG
;
- Publication Type:Journal Article
- Keywords:
pulmonary artery;
smooth muscle cells;
Janus kinase protein;
immunocytochemistry
- From:Journal of Third Military Medical University
1984;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of hypoxia on the expression of Janus kinase (JAK) in cocultured pulmonary arterial smooth muscle cells (PASMC) Methods Rat PASMC were cocultured with lung microvascular endothelial cells (LMVEC), the expression of JAK protein in PASMC was detected with Immunocytochemical method Results Expressions of JAK1 and JAK3 Protein were seen excessive positive (++++) in the cytoplasm of simple PASMC in hypoxia, and better positive (+++) in cocultured PASMC in hypoxia Compared with those of the control group, the expressions of JAK1 and JAK3 protein obviously increased in PASMC in hypoxia for 2 h, reached highest in those with hypoxia for 6 h, and decreased in those with hypoxia for 12 hours hypoxia, but still higher than those in hypoxia for 2 h Conclusion Hypoxia modulates the signal pathways and improves cell proliferation by the enhancement of the expressions of JAK1 and JAK2 proteins in co cultured PASMC
