Effects of NMDA receptor on glucocorticoid receptor mRNA levels in hippocampus of rats after scald burn stress
- VernacularTitle:NMDA受体对烫伤应激大鼠海马糖皮质激素受体基因表达的影响
- Author:
Jianhua LU
;
Jian DANG
;
Haidi LI
;
Jingsheng GAO
;
Jiaxiang XIONG
;
- Publication Type:Journal Article
- Keywords:
burns;
stress;
hippocampus;
NMDA receptors;
glucocorticoid receptors;
gene expression
- From:Journal of Third Military Medical University
1984;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes of glucocorticoid receptor (GR) gene expression in the hippocampus of rats following scald burn stress and the role of N methyl D aspartate (NMDA) receptor in this change. Methods Adult male Wistar rats inflicted with 30% TBSA full thickness scalding were applied as severe scalding stress model. GR mRNA levels in the hippocampus were detected with RT PCR. Results A significant decrease of GR mRNA levels was observed in the hippocampus 2 h after the scalding stress. The decrease could be inhibited when MK 801, an NMDA receptor antagonist, was administered prior to stress, and be augmented with the administration of NMDA, an NMDA receptor agonist, but not be affected by normal saline. Conclusion NMDA receptors are involved in the scalding stress induced down regulation of GR gene expression in the rat hippocampus.
