Role of intracellular calcium in proliferation inhibition of MCF-7 cells induced by HMG-CoA reductase inhibitor
- VernacularTitle:细胞内Ca~(2+)在HMG-CoA还原酶抑制剂抑制MCF-7细胞增殖中的作用
- Author:
Yong ZHOU
;
Mantian MI
;
Na WEI
;
Zhixiang YANG
;
- Publication Type:Journal Article
- Keywords:
lovastatin;
human brest cancer cell;
HMG CoA reductase inhibitor;
cell cycle;
cholesterol;
[Ca 2+ ]i;
plasma membrane calcium ATPase
- From:Journal of Third Military Medical University
1984;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of HMG CoA reductase inhibitor, lovastatin (LOV), on the proliferation of human breast cancer cell MCF 7 and the role of intracellular calcium concentration ([Ca 2+ ]i) in this event. Methods After treated with LOV at the dosages of 4, 8 and 16 ?mol/L for 1-3 d respectively, the proliferation of MCF 7 cells was examined with MTT, and the distributions of cell cycles with FCM assay. Meanwhile, the change of [Ca 2+ ]i of MCF 7 cells was observed with laser scanning confocal microscopy, and the expression of plasma membrane calcium ATPase isoform 1 (PMCA1) mRNA with RT PCR. Results Lovastatin, inhibited the proliferation of MCF 7 cells and arrested MCF 7 cells in the G 0/G 1 phase of cell cycle, in a dose and time dependent manner. However, apoptosis of LOV treated MCF 7 cells was not obvious. Simultaneously, LOV increased [Ca 2+ ]i of MCF 7 cells, but didn't change the expression of PMCA1 mRNA. Conclusion The results suggest that LOV has the capabilities of inhibiting the proliferation of MCF 7 cells and arresting them in G 0/G 1 phase. These effects of LOV maybe correlate with LOV changing the function of PMCA1and increasing [Ca 2+ ]i of MCF 7 cells.
