Effect of procyanidins on carcinogen-induced DNA damage and topoisomerase
- VernacularTitle:原花青素对MNNG致DNA损伤及拓扑异构酶Ⅱ的影响
- Author:
Yin LU
;
Zhiguang SUN
;
Wenxing CHEN
;
Aiyun WANG
;
Ling LI
;
Quan ZHU
;
- Publication Type:Journal Article
- Keywords:
procyanidins;
DNA damage
- From:
Chinese Pharmacological Bulletin
1987;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To observed the effect of procyanidins on DNA damage induced by carcinogen or hydroxyl radical (OH?),and inhibitory effect on topoisomerase. METHODS Using labeled TdR uptake assay and single cell gel electrophonesis. Topoisomerase was extracted from B16F10 cell lines, used agarose gel electrophoresis assay. RESULTS It was showed that hydroxyl radical (OH?) or different doses of MNNG can induce severe DNA damage of L 929 cell lines. Procyanidins can alleviate the damage induced by MNNG or OH?. It shows certain dose dependent effect relationship . But there is no significant inhibitory against Topo Ⅱ activity by procyanidins even at a dose of 50 ?mol?L -1 . CONCLUSION Procyanidins from grapes seeds can protect DNA damage,but not inhibit topoisomerase activity. This is one of cancer chemoprevention mechanism of Procyanidins.
