Effects of insulin-like growth factor on expression of osteopontin in rat vascular smooth muscle cells
- VernacularTitle:IGF对大鼠平滑肌细胞骨桥蛋白表达的影响
- Author:
Min HU
;
Jinde YU
;
- Publication Type:Journal Article
- Keywords:
rat vascular smooth muscle cells;
osteopontin;
IGF I;
gene expression
- From:
Chinese Pharmacological Bulletin
1987;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To explore whether or not the function of insulin like growth factor (IGF I) in promoting osteopontin gene expression and protein synthesis of rat vascular smooth muscle cell (VSMC). METHODS Cultured cells were incubated with different concentration of IGF I for different hours, then RT PCR and Western blotting analysis were used to observed the effects of IGF I on osteopontin expression of rat vascular smooth muscle cells RESULTS IGF I (20 ?g?L -1 ) could obviously induce the gene expression and protein synthesis of osteopontin, RT PCR results showed that the ratio of mRNA of osteopontin and ? actin in cells incubated with IGF I (20 ?g?L -1 ) for 3, 24 and 48 h increased by 39 70%,40 44% and 54 05% respectively, as compared with that of the control ( P
