Enhancement of cytotoxicity of Adramycin by neferine in Saos-2 cells and its mechanism
- VernacularTitle:甲基莲心碱增强阿霉素对骨肉瘤的抑制作用及机制初探
- Author:
Jie BAO
;
Jiangnan ZHOU
;
Xiaoqing TANG
;
Jianguo CAO
;
- Publication Type:Journal Article
- Keywords:
neferine;
adriamycin;
chemosenstizer;
Rb protein;
apoptosis;
clee cycle
- From:
Chinese Pharmacological Bulletin
1987;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To study the synergistic anticancer effccts of neferine combined with anticancer agents on Saos 2 cell lines and its mechanism. METHODS\ Cytotoxicity assay was tested by MTT method.Cell cycle and apoptosis was detected by PI staining flow cytometty. Expression of Rb protein was examined by indirect immune fluorescent symbol flow cytometty and S P immunohistochemical staining. RESULTS\ 5,10 ?mol?L -1 Nef augmented apoptosis of Saos 2 cells induced by ADM,enhanced the expression of Rb protein in Saos 2 cells and raised the G 1 phase ratio of Saos 2 cells. CONCLUSION Nef can promote chemotherapy sensitivity to ADM in Saos 2 cells. Its mechanism is associatd with the up modulation of Rb protein expression in the Saos 2 cells arrest cells in G 1 phase
