Effects of fetal bovine serum on differentiation of human fetal neural stem cells in vitro
- VernacularTitle:胎牛血清对人胚胎神经干细胞分化的影响
- Author:
Wei LI
;
Chengren LI
;
Wenqin CAI
;
Zhongxiang YAO
;
- Publication Type:Journal Article
- Keywords:
neural stem cell;
fetal bovine serum;
human
- From:Journal of Third Military Medical University
1984;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of fetal bovine serum(FBS) on differentiation of human neural stem cells (NSCs). Methods The effects of FBS with different concentrations on differentiation of human fetal NSCs were observed by cell culture, immunocytochemistry and flow cytometry. Results Human fetal NSCs could be induced to differentiate mainly three types of nerve system cells(neuron, astrocyte and oligodentrocyte). There were 80%～90% astrocytes of differentiated cells from human fetal NSCs with the concentration of 15% FBS induced. Conclusion Concentration dependent FBS in culture medium may have effect on the ratio of neurons to glial cells differentiated from human NSCs in vitro .
