Design of antisense drugs targeting bcl-2 mRNA and effect of them on apoptosis of leukemia cells
- VernacularTitle:bcl-2反义药物的设计及其对白血病细胞生长的影响
- Author:
Xiaoyong LEI
;
Huan ZHANG
;
- Publication Type:Journal Article
- Keywords:
bcl 2 oncogene;
antisense oligodeoxynucleotide;
drug design;
leukemia cell
- From:
Chinese Pharmacological Bulletin
1986;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM The antisense drug design will be optimized based on bcl 2 mRNA secondary structure simulated with computer. METHODS bcl 2 mRNA second structures were simulated with computer and Mfold software, and the unstable zones on the second structure, as designing antisense zones, were selected. RESULTS Five antisense deoxynucletides were studied and evaluated with experiments of HL 60 and K562 leukemic cells. Two of them exist significant effect of inhibiting grow of HL 60 and K562 leukemic cells with dose of 10 ?mol?L -1 or more. CONCLUSION The designs with computer and corresponding software will be usefully efficient way to look for antisense drugs.
