R-HPLC determination of plasma AZT concentration
- VernacularTitle:反相高效液相色谱法检测齐多夫啶的血药浓度
- Author:
Shu GAO
;
Jun LI
;
Longfu RONG
;
Yong JIN
;
Hongwei YAO
;
Lin GUO
;
Shuyun XU
;
- Publication Type:Journal Article
- Keywords:
HPLC;
zidovudine;
plasma concentration
- From:
Chinese Pharmacological Bulletin
1986;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish a RP HPLC method for the determination of zidovudine(AZT) plasma concentration. METHODS A shim pack,VP ODS. 5 ?m 4 6?150 mm radial compression column was used with a mobile phase of tetrahydrofuran water trifluoroacetic acid(10∶89 8∶0 2). The flow rate was 1 0 ml?min -1 .The detector was operated at 267 nm. RESULTS The calibration curves of AZT were linear separately within the concentration range of 0 02～20 mg?L -1 . The relative standard deviations for within day and between days assays were all less than 5%. The relative recovery of the method were 96 4%. The limit of quantitation of the assay is 10 ?g?L -1 of plasma. CONCLUSIONS The methed is rapid,accurate and stable. The determination of AZT plasma concendration could be used.
