Lipopolysaccharide induced NO production in synoviocytes and inhibitory effect of genistein on it
- VernacularTitle:脂多糖诱导滑膜细胞产生一氧化氮及木黄酮对其的抑制作用
- Author:
Jiesong WANG
;
Junping ZHANG
;
Min ZHANG
;
Xingqi TAN
;
Dinghua QIAN
;
- Publication Type:Journal Article
- Keywords:
genistein;
lipopolysaccharides;
nitric oxide;
synoviocytes
- From:
Academic Journal of Second Military Medical University
1999;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effect of lipopolysaccharides(LPS) on nitric oxide(NO) production in synoviocytes and inhibition of genistein on it.Methods: LPS or LPS+genistein stimulated synoviocytes in vitro .NO in culture medium was determined by Griess method.Results: LPS dose and time dependently induced NO production in synoviocytes; Genistein (6.25 50 ?mol/L) dose dependently inhibited the production of NO induced by lipopolysaccharide.Conclusion:The effect of LPS on NO production can be dose dependently inhibited by genistein,this may be mediated by tyrosine kinase(PTK).
