Studies on a high-throughput screening method for identification of HIV fusion inhibitors targeting gp41
- VernacularTitle:作用于gp41的HIV融合抑制剂高通量筛选方法的研究
- Author:
Shuwen LIU
;
Shibo JIANG
;
Beiyi LIU
;
Zhihua WU
;
Lin LV
;
Jiajie ZHANG
;
Ning FU
;
Shuguang WU
;
- Publication Type:Journal Article
- Keywords:
HIV gp41;
ELISA;
high throughput screening method;
HIV fusion inhibitors;
anti HIV agents
- From:
Chinese Pharmacological Bulletin
1986;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To modify and improve a screening assay so that it becomes more convenient, economic and adaptable in China for high throughput screening of HIV fusion inhibitors targeting gp41. METHODS The original screening method reported by Jiang et al (J Virol. Methods 1999;80:85 96) was modified by: ① using a conformation specific monoclonal antibody to replace a polyclonal antibody for coating plates; ②simplifying the procedures; ③using parts of the reagents produced in China. RESULTS The modified screening assay is simpler, more convenient, and more economic than the original assay, but its sensitivity is comparable to and specificity is a little better than the original method. CONCLUSIONS The modified screening assay is more convenient and economic and can be used in China for high throughput screening of HIV fusion inhibitors from complex sample, such as phage display peptide libraries, microorganism fermentation liquids, herbs and other natural products.