Preparation, identification and biological activity of rhIL-2/GM-CSF fusion protein antibodies
- VernacularTitle:rhIL-2/GM-CSF融合蛋白抗体制备、鉴定及其生物学活性研究
- Author:
Xingmei LINLAI
;
Mingqian ZHOU
;
Zehong CHEN
;
Yali ZHANG
;
Xiaoning WANG
;
- Publication Type:Journal Article
- Keywords:
interleukin 2(IL 2);
granulocyte macrophage colony stimulating factor(GM CSF);
fusion protein;
antibody
- From:
Academic Journal of Second Military Medical University
2000;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To prepare and identify recombinant human IL 2/GM CSF(rhIL 2/GM CSF) fusion protein antibodies and to study its specificity and its effect on fusion protein biological activity. Methods: rhIL 2 /GM CSF fusion protein was purified by DEAE Sepharose FF ion exchange chromatography. The purified protein was used to immunize rabbits for the preparation of antisera. The titer and specificity of the antisera were detected by ELISA and Dot ELISA and the biological activity by cell proliferation. Results: The antisera not only reacted with the rhIL 2/GM CSF, IL 2 and GM CSF, but also inhibited the biological activity of the rhIL 2/GM CSF, IL 2 and GM CSF. Conclusion: The obtained antisera can be used to study the structure and function of the rhIL 2/GM CSF.
