Possible mechanism of mitochondria on anti-apoptotic effect of ginsenoside Rg1 on MPP~+-induced cellular apoptosis
- VernacularTitle:抗氧化作用可能是人参皂甙Rg1抗细胞凋亡的机制
- Author:
Fang FANG
;
Xiaochun CHEN
;
Yuangui ZHU
;
- Publication Type:Journal Article
- Keywords:
ginsenoside;
MPP +;
SHSY5Y cells;
apoptosis
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2002;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the possible anti apoptotic mechanism of ginsenoside Rg1 on MPP + induced cellular apoptosis. METHODS: The apoptosis of SHSY5Y induced by MPP + was observed by AO EB staining. Flow cytometry was used to quantitate mitochondrial transmembrane potential (△?m) and reactive oxygen species (ROS) . Western Blot was used to detect the expression of bcl 2 and bax proteins in SHSY5Y cells. RESULTS: MPP + induced apoptosis in SHSY5Y cells was obviously inhibited by pretreatment with 10 ?mol?L -1 Rg1. Although there was no difference of mitochondrial transmembrane potential between Rg1 pretreated groups and MPP + groups, the level of ROS in Rg1 pretreated groups decreased, the expression of bcl 2 protein increased and expression of bax protein decreased. CONCLUSION: Rg1 protects against MPP + induced apoptosis in SHSY5Y cells and the effect may be attributed to its remove of ROS and its regulation of expression of bcl 2 and bax proteins.
