Influence of nicotinylmethylamide on the pharmacokinetic parameters of cyclosporin A by HPLC
- VernacularTitle:高效液相色谱法测定羟甲烟胺对环孢菌素A药物动力学参数的影响
- Author:
Qin LI
;
Yan ZHAO
;
Yongguang LIANG
;
Shaofen TANG
;
Ruichen GUO
;
- Publication Type:Journal Article
- Keywords:
cyclosporin A;
nicotinylmethylamide;
HPLC;
pharmacokinetics
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2002;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To establish a simple, rapid and accurate high performance liquid chromatographic method (HPLC) for the determination of cyclosporin A (CsA) in human whole blood, and to study the influence of nicotinylmethylamide (Nic) on the pharmacokinetic parameters of cyclosporin A (CsA). METHODS: Whole blood CsA concentrations were measured by HPLC in 18 healthy volunteers administrated single CsA or co administrated Nic. The data of time blood concentrations of CsA were analyzed by 3p97 Program. The analysis of variance and two one sided t test were used to compare the main pharmacokinetic parameters of CsA in the two administrations. RESULTS: C max and AUC 0～∞ of CsA had statistically significance between the single CsA group and co administration of Nic group (P0.05). CONCLUSION: This HPLC method is simple, sensitive and accurate, and is suitable for routine determination of blood CsA levels in human. Nic can improve the absorption of CsA and increase the C max and AUC of CsA, but has no influence on the metabolism of CsA.
