EFFECTS OF POLYENE FATTY ACID ON FATTY ACID COMPOSITION, GROWTH AND DEVELOPMENT OF HIPPOCAMPAL NEURONS
- VernacularTitle:多烯脂肪酸对海马神经元细胞脂肪酸构成和生长的作用
- Author:
Chaogang CHEN
;
Yixiang SU
;
Bingyan TAN
;
Xiang FENG
;
Jian WEN
;
- Publication Type:Journal Article
- Keywords:
arachidonic acid;
docosahexaenoic acid;
hippocampus/cytology
- From:
Acta Nutrimenta Sinica
1956;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effects of long chain PUFA, AA and/or DHA on the fatty acid composition, growth and development of the newborn rats hippocampal neurons in culture. Methods: The primary culture of the hippocampal neurons was carried out in vitro by using serum free medium and supplementing 4 ? mol/L AA, DHA, or AA and DHA, of which the total concentration were 4 ?mol/L, the ratios of AA∶DHA from 1∶2 to 16∶1, in medium. The hippocampal neuronal fatty acid composition was analyzed by gas chromatogram, and the neuronal size and length of hippocampal neurite were measured by image analysis. Results: There were significant positive correlations between the ratios AA∶DHA in medium and the AA percentage and the ratios AA∶DHA in hippocampal neurons. The soma area? body maximum? minimum diameter and process length of hippocampal neurons in the group, which the total concentration of AA and DHA was 4 ?mol/L, and the ratio AA∶DHA in medium was 2∶1 or 4∶1,were higher significantly than other groups and groups with other ratios of AA∶DHA. Conclusion: AA and DHA could promote the growth and development of hippocampal neurons in culture..
