Thalidomide inhibiting growth and metastasis of human gastric cancer implanted in nude mice
- VernacularTitle:沙利度胺对裸鼠原位种植人胃癌生长和转移的抑制作用
- Author:
Guofeng ZHANG
;
Yuanhe WANG
;
Qiang WANG
;
- Publication Type:Journal Article
- Keywords:
stomach neoplasms;
neoplasm metastasis;
thalidomide;
neovascularization, pathologic;
apoptosis
- From:
Academic Journal of Second Military Medical University
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the inhibition effects of thalidomide on the growth and metastasis of gastric cancer in vivo in nude mice. Methods: Metastatic model simulating human gastric cancer was established by orthotopic implantation of histologically intact human tumor tissue into gastric wall of nude mice. Mice were randomly divided into 4 groups: control group (saline solution 0.5 ml, ip), 5 FU group (fluorouracil 30 mg?kg -1 ?d -1 , ip), thalidomide group (thalidomide 250 mg?kg -1 ?d -1 ,ip), combined treatment group (both 5 FU and thalidomide, ip). Six weeks after implantation, the tumor weight, inhibition rates, intratumoral microvessel density (MVD), apoptotic index (AI) and the metastasis were evaluated after the mice were sacrificed. Results: Compared with the control group, growth of tumor was significantly reduced in mice treated with 5 FU, thalidomide and combined treatment (inhibition rate 39.8%, 48.1% and 74.1%). The incidences of liver metastases was also significantly inhibited in the 5 FU group, thalidomide group and combined treatment group than in control group(8/11 vs 4/12, 3/12 and 0/12). The incidences of peritoneal metastases was also significantly inhibited in the 5 FU group, thalidomide group and combined treatment group than in control group(7/11 vs 3/12, 3/12 and 0/12). The MVD decreased significantly in thalidomide group and combined treatment group. AI increased significantly in the treated mice. Conclusion: Thalidomide can induce apoptosis in gastric cancer by inhibiting tumor angiogenesis and has inhibitory effect on growth and metastasis of human gastric cancer implanted in nude mice. Combination of thalidomide with cytotoxic agents is more effective.
