Effects of endothelin-1 on the expression of ClC-3 chloride channel in bovine cerebrovascular smooth muscle cells
- VernacularTitle:内皮素-1对牛脑血管平滑肌细胞ClC-3通道表达的影响
- Author:
Linxi CHEN
;
Yongyuan GUAN
;
Hua HE
;
- Publication Type:Journal Article
- Keywords:
chloride channel;
ClC 3;
cerebrovascular;
smooth muscle cell;
endothelin 1
- From:
Chinese Pharmacological Bulletin
1987;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To study the effects of endothelin 1(ET 1) on ClC 3 chloride channel protein expression in cultured bovine cerebrovascular smooth muscle cells (CSMC). METHODS Cell culture and Western blot. RESULTS ① The endogenous ClC 3 expression was found in basilar artery, middle cerebral artery, and microvessel; ② The molecular weight of expressed ClC 3 chloride channel protein was about 95 ku; ③ ET 1 enhanced ClC 3 protein expression which was inhibited by nifedipine and SK&F96365. Cyclopiazonic acid(CPA) increased the expression of ClC 3 protein in a concentration dependant manner, and enhanced ET 1 effect on this protein expression. CONCLUSION ET 1 stimulated ClC 3 chloride channel protein expression in cultured bovine cerebrovascular smooth muscle cells. The intracellular Ca 2+ plays an important role on signal transduction pathway in ClC 3 protein expression process.
