Inhibition of proliferation and apoptosis in HL60 cells induced by cobra venom serum
- VernacularTitle:中华眼镜蛇毒血清抑制HL60细胞增殖及诱导凋亡的实验研究
- Author:
Yingru LUO
;
Chunling YE
;
Xianda REN
;
Hongliang LI
;
Ling ZHONG
;
- Publication Type:Journal Article
- Keywords:
cobra venoms;
leukemia;
apoptosis
- From:
Chinese Pharmacological Bulletin
1987;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To explore the effects and mechanism of cobra venom serum on the proliferation in HL60 cells. METHODS Established the HL60 cells as a target to study the growth feature by the action of cobra venom serum.The agarose gel electrophoresis and flow cytometry analysis were used to demonstrate apoptosis. RESULTS Compared with the control group, the cells were inhibited significantly by the action of cobra venom serum.A characteristic DNA "ladder" was detected by using agarose gel electrophoresis. By flow cytometry analysis,it was proved that most apoptosis of HL60 cells occurred when cultured with cobra venom serum. CONCLUSION Cobra venom serum inhibited the HL60 cells in vitro , which was related to apoptosis. This may introduce a new way to the treatment of leukemia.
