EFFECTS OF PROCYANIDINS EXTRACT FROM THE LOTUS SEEDPOD ON REACTIVE OXYGEN SPECIES AND LIPID PEROXIDATION
- VernacularTitle:莲房原花青素对氧自由基和脂质过氧化的作用
- Author:
Zhiqun LING
;
Bijun XIE
;
- Publication Type:Journal Article
- Keywords:
Nelumbo nucifera Gaertn;
seadpod of the lotus;
procyanidins;
free radical;
Lipid peroxide;
superoxide dismutase;
glutathione S transferase
- From:
Acta Nutrimenta Sinica
1956;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effects of procyanidins extract from the lotus seedpod (LSPC) on active oxygen radicals and lipid peroxidation. Methods: (1) The scavenging effects of LSPC on O ?- 2 and ?OH was investigated by using chemiluminescence method; (2) The effects of different concentrations of LSPC on RBC auto oxidation and LPO formation of rat liver mitochondria in vitro were observed; (3) The LPO level of liver homogenates incubated with Fe 2+ and H 2O 2 in vitro were determined; (4) the mice were treated with a single intragastric feeding of 5% CCl 4 soya bean oil solution (10ml/kg bw) after seven days supplementation of LSPC 100, 200, 400 mg/(kg bw?d). The activities of SOD, GST and the level of LPO in liver and plasma of CCl 4 toxic mice were determined. Results: In vitro, LSPC 169.0mg/L or 105.3mg/L was shown to markedly scavenge O ?- 2 produced in xanthine/xanthine oxidase and ?OH in Fenton systems respectively, and could significantly inhibit the formation of liver LPO. In vivo, LSPC (100,200mg/kg) evidently reduced the level of LPO and increase the activities of SOD and GST. Conclusion: It suggests that LSPC may be used as an antioxidant and might effectively protect liver from the injury due to lipid peroxidation.
