DETERMINATION OF BIOTIN IN FOODS AND FEEDS
- VernacularTitle:食物和饲料中生物素的测定方法
- Author:
Lei SHI
;
Xiaoli YANG
;
Guodong WANG
;
Yuexin YANG
;
- Publication Type:Journal Article
- Keywords:
food;
feed;
biotin;
microbiological method
- From:
Acta Nutrimenta Sinica
1956;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish microbiological method for determining biotin in foods and feeds. Methods: Using biotin dependent microorganisms Lactobacillus plantarum(ATCC 8014), the biotin content was detected indirectly by the growth of cultrued bacteria spectrophotometrically.Results: The detection limit was 0.03 ng. The relative standard deviations(RSD) of within and between run assays were 2.2%-3.8%,2.1%-5.3% respectively. The recovery of added biotin was 93.4%-104.6%, and RSD was 3.1%-4.1%. Conclusion: This assay is sufficiently accurate and repoducible for determining biotin in foods and feeds.
