Study of molecular mechanism of cardiac injury induced by adriamycin
- VernacularTitle:盐酸阿霉素对大鼠心肌损伤的分子机制研究
- Author:
Yongmei LI
;
Bogen SONG
;
Guifen ZHAO
;
Lixian SHEN
;
- Publication Type:Journal Article
- Keywords:
adriamycin;
Cu Zn SOD;
GSH Px;
fibronectin;
P105;
gene expression;
heart;
rats
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2000;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To preliminarily study the molecular mechanism for the cardiac injury in rat by adriamycin and the mechanism for the acute repair in the body. METHODS: The male Sprague Dawley rats were randomly divided into four groups (n=10 in each): The first group was kept without treament and served as the control ;the second, the third and the fourth received ADR in different doses (10, 20, 40 mg?kg -1 , respectively) by injection of adriamycin. The content of malondialdehyde (MDA)in the serum was estimated with thiobarbituric acid. Cu Zn SOD was measured by its reaction with xanthine oxidase. GSH was measured by its reaction with 5, 5 nitrobenzoic acid. Using semi quantitative reverse transcription polymerase chain reaction (RT PCR),we analyzed the expression of the associated gene. RESULTS: MDA contents in the medium and high ADR dose groups were higher than that in the control group (P
