Regulation of telomerase activity and cell cycle by matrine in hepatoma cells in vitro
- VernacularTitle:苦参碱对肝癌细胞端粒酶活性调控及细胞周期的影响
- Author:
Weizhong CHEN
;
Yong LIN
;
Weifen XIE
;
Junping ZHANG
;
Xin ZHANG
;
Zhihong CHENG
;
- Publication Type:Journal Article
- Keywords:
matrine;
telomerase;
cell cycle;
gene expression regulation
- From:
Academic Journal of Second Military Medical University
1985;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of matrine (Ma) on telomerase activity and cell cycle in hepatoma cell line HepG 2 cells. Methods: TRAP ELISA method was used to determine the telomerase activity in HepG 2 cells which were treated with different concentrations of Ma. Plasmid inserted with 800 bp of the human telomerase reverse transcriptase (hTERT) promoter was transiently transfected into HepG 2 cells by lipofect. Different concentrations of Ma were added into culture media 2 h later, and the activity of the hTERT promoter was tested 48 h after transfection. In addition, the percentages of HepG 2 cells in different cell cycle were determined by the flow cytometry on the 24, 48 and 72 h respectively after adding the different concentrations of Ma. Results: The telomerase activity of HepG 2 was suppressed by Ma at the dose of 750 ?g/ml and the expression of hTERT promoter was also inhibited. The percentage of G 0/G 1 stage cells increased and the percentage of S and G 2/M stages cells decreased in both 500 ?g /ml and 750 ?g /ml groups 48 and 72 h after Ma was added. Conclusion: Ma may have inhibitory effect on hTERT promoter expression, which is related to the telomerase activity and cell cycle regulation.
