The role of survivin on the apoptosis of lung cancer cell line induced by chemotherapeutical drugs
- VernacularTitle:survivin基因在化疗药物诱导的肺癌细胞凋亡中的作用
- Author:
Xiaoyang WANG
;
Zhenxiang ZHANG
;
Xirong LI
;
- Publication Type:Journal Article
- Keywords:
survivin;
apoptosis;
lung neoplasms;
cisplatin;
etoposide
- From:
Chinese Pharmacological Bulletin
1987;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To study the role of survivin on the apoptosis of lung cancer cell line induced bycisplatin (DDP) and etoposide (VP16). METHODS The lung adenocarcinoma cell line A549 was chosen in this experiment. The inhibitory effects of chemotherapeutical drugs on A549 cell line were assayed with MTT test. Cultured cells was divided into 3 groups: DDP, VP16 and control. Both DDP and VP16 group were divided into high concentration group and low concentration group, respectively. Expression of survivin gene were detected by reverse transcriptase PCR. Apoptosis was deterinined by flow cytometry. RESULTS Both DDP and VP16 obviously inhibited the lung cancer cell growth with dose and time dependent. Apoptosis and inhibitory rate of survivin gene expression induced by chemotherapeutical drugs was higher than that of control group. The effects also were time dependent. CONCLUSION The inhibition of survivin gene expression may play a critical role on the lung cancer cell apoptosis induced by DDP and VP16.
