Effects of tyrosine kinase inhibitor on ?_(1B)-AR-induced Ca~(2+)influx involved in hTrp3 protein
- VernacularTitle:酪氨酸激酶抑制剂对hTrp3蛋白介导的?_(1B)-AR引起的Ca~(2+)内流的影响
- Author:
Xiaoru YANG
;
Yongyuan GUAN
;
Qinying QIU
;
Hua HE
;
Jinliang LI
;
- Publication Type:Journal Article
- Keywords:
? 1B AR;
ROC;
Trp;
tyrosine kinase;
genistein
- From:
Chinese Pharmacological Bulletin
1987;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To investigate the role of Trp3 in the Ca 2+ influx induced by ? 1B AR in HEK293 cells and the effect of tyrosine kinase on it. METHODS With lipofect AMINE2000 reagent, hTrp3 cDNA was transfected to HEK293 cells and ? 1B HEK293 cells respectively. The expression of Trp3 was examined by Western blot. With Fura 2/AM spectrophoto fluorometry, Ca 2+ influx was determined. RESULTS HTrp3 was expressed endogenously in HEK293 cells, and the expression increased in hTrp3 transfected cells. Compared with untransfected cells, transfection of hTrp3 cDNA increased Ca 2+ influx induced by ? 1B AR ( P 0 05). 5～30 ?mol?L -1 genistein inhibited Ca 2+ influx induced by ? 1B AR in hTrp3 cDNA transfected cells and the maximum inhibitory rate was (75 2?12 6)% . CONCLUSION Transfection of hTrp3 cDNA increased Ca 2+ influx induced by ? 1B AR in HEK293 cells. This process was regulated by tyrosine kinase.
