Effect of tunicamycin-inhibitor of N-oligosaccharide chain on glomerular mesangial cells
- VernacularTitle:N-糖链抑制剂衣霉素对肾小球系膜细胞的影响
- Author:
Songmin HUANG
;
Haiyan ZHOU
;
Li XIAO
;
Al EL
;
- Publication Type:Journal Article
- From:
Chinese Journal of Nephrology
1994;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of tunicamycin(TM)-inhibitor of N-glycosylation of proteins on proliferation and apoptosis of glomerular mesangial cells(GMC) and synthesis of FN. Methods Morphology of GMC apoptosis was observed under eletron microscopy, and flowcytometry was used for assessing apoptosis rate. GMC proliferation was examined by MTT assay. The amount of FN was measured by ELISA. Results TM inhibited the proliferation of GMC as compared with untreated controll ( P
