Clinical significance of dendritic cell infiltration and surface molecules expression in gastric cancer
- VernacularTitle:胃癌组织中树突状细胞浸润及其表面分子表达的临床意义
- Author:
Zhengxin WANG
;
Minghui ZHANG
;
Nan LI
;
Yuanhe WANG
;
Qiang WANG
;
Xuetao CAO
;
- Publication Type:Journal Article
- Keywords:
dendritic cells;
stomach neoplasms;
neoplasm staging;
membrane proteins
- From:
Academic Journal of Second Military Medical University
1981;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the clinical significance of dendritic cell(DC) infiltration and molecules expression in gastric cancer.Methods:After tumor mass was digested by enzyme and cells were seperated,anti S 100 protein antibody immunohistochemical staining and flow cytometric analysis were done in 15 cases of gastric cancer.Reverse transcription polymerase chain reaction(RT PCR)was used to detect IL 10,VEGF and TGF ? 1 mRNA expression in gastric cancer cells.Results:The number of DC in gastric cancer was just small, and correlated reversely with pathologic stages.The levels of some surface molecules expression such as MHC class Ⅱ, costimulatory molecules B7 1 and ICAM 1 in gastric cancer were negative.The number of DC and the levels of some surface molecules expression in gastric cancer were correlated reversely with pathologic stages.Levels of IL 10,VEGF and TGF ? 1 mRNA expression in gastric cancer cells were high.Conclusion:The results indicate that infiltration and surface molecules expression in gastric cancer are closely linked to tumor pathologic stages.
