Behavior changes and expression of nitric oxide synthase mRNA of hypothalamus after sleep deprivation in rats
- VernacularTitle:大鼠睡眠剥夺后行为及下丘脑诱生型一氧化氮合酶mRNA表达的变化
- Author:
Changyun LIU
;
Haiming WANG
;
Jian CHEN
;
Hongguang JI
;
Jianguang ZHOU
;
- Publication Type:Journal Article
- Keywords:
sleep deprivation;
sleep,REM;
behavior;
nitric oxide synthase;
hypothalamus
- From:
Academic Journal of Second Military Medical University
1981;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effects of rapid eye movement sleep (REMS) deprivation on active behavior and the expression of mRNA coding for inducible nitric oxide synthase (iNOS) of hypothalamus in rats. Methods: The flower pot technique was adopted to deprive the REMS of Sprague Dawley rats for 24 h, 48 h and 72 h respectively. The active behavior of rats were examined by the open field test (OFT). The expression of mRNA coding for iNOS of hypothalamus in rats was assayed by reverse transcription polymerase chain reaction (RT PCR). Results: The OFT scores were significantly increased in rats subjected to 24 h and 48 h REMS deprivation ( P 0.05). There was no difference in the expression of iNOS mRNA among 24 h, 48 h REMS deprivation and the control groups, but the expression was significantly increased in 72 h REMS deprivation group ( P
