Tetracycline inhibits the expression of adhesive molecules on activated endothelial cells
- VernacularTitle:四环素抑制活化的内皮细胞表达粘附分子
- Author:
Yan XIONG
;
Zhimin HE
;
- Publication Type:Journal Article
- Keywords:
tetracycline;
tumor necrosis factor-alpha;
lipopolysaccharide;
human umbilical vein endothelial cells;
E-selectin;
intercellular adhesion molucule-1
- From:
Chinese Pharmacological Bulletin
1987;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To investigate the effects of tetracycline on the expression of adhesive molecules E selectin and intercellular adhesion molecule 1 (ICAM 1) on human umbilical vein endothelial cells (HUVECs) stimulated by tumor necrosis factor alpha (TNF ?) or lipopolysaccharide (LPS). METHODS HUVECs were activated by TNF ? (0 5～50 ?g?L 1 ) or LPS (0 5～50 mg?L -1 ). Tetracycline (10 -6 ～10 -4 mol?L -1 ) was used to incubate HUVECs for 1 h before stimulation with TNF ? ( 1 ?g?L -1 ) or LPS ( 5 mg?L -1 ) and co incubate HUVECs with TNF ? or LPS for 4 h or 18 h. The expression of adhesive molecules on HUVECs was measured by a cellular enzyme linked immunoabsorbent assay (Cell ELISA) method. RESULTS After stimulation of HUVECs with TNF ? or LPS for 4 h induced E selectin expression, and exposure HUVECs to TNF ? or LPS for 18 h increased constitutive expression of ICAM 1 compared with control group ( P
