Combined inhibitory effects of propofol and midazolam on macroscopic sodium currents in rat sympathetic ganglion neurons
- VernacularTitle:异丙酚、咪达唑仑抑制交感神经节细胞钠通道电流的联合作用
- Author:
Jijian ZHENG
;
Xinliang ZHUANG
;
Dongping DU
;
Qinghong MAO
;
Guohui XU
;
- Publication Type:Journal Article
- Keywords:
anesthesia;
propofol;
midazolam;
sympathetic ganglion;
patch clamp/sodium channel;
drng interaction
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2000;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the interactions of propofol and midazolam on the whole cell sodium channel currents in rat sympathetic ganglion neurons. METHODS: Whole cell patch clamp recordings were made from enzymatically isolated rat (7- 10 d ) superior cervical sympathetic ganglion neurons. Isobolographic analysis was applied to evaluate the potency of combinations of propofol and midazolam on Na + channel currents. RESULTS: Under V h= 80 mV and V t= 0 mV . Propofol and midazolam dose dependently blocked Na + currents with a mean drug concentration required to produce 50% current inhibition (IC 50 ): 33.12 ?mol?L -1 and 18.35 ?mol?L -1 ; clinically relevant concentrations of propofol and midazolam reduced Na + peak currents by 27.66 % (P
