Inhibitive effects of genistein and quercetin on NIH3T3 fibroblast proliferation and collagen synthesis
- VernacularTitle:染料木素和槲皮素对成纤维细胞增殖和胶原合成的抑制作用
- Author:
Lihong QI
;
Luping KANG
;
Junping ZHANG
;
Futang LIU
;
Jianping ZHOU
;
Bin ZHOU
;
- Publication Type:Journal Article
- Keywords:
genistein;
quercetin;
fibroblast;
collagen
- From:
Academic Journal of Second Military Medical University
1999;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of genistein (GE) and quercetin (QU) on proliferation and collagen synthesis of mouse fibroblast NIH3T3 cells.Methods:Cell proliferation was measured by crystal violet staining assay.Collagen synthesis was determined by 3H proline incorporation assay.Results:GE (25 70 ?mol?L -1 ) and QU (6.25 50 ?mol?L -1 ) concentration dependently reduced the serum driven increase of cell proliferation and collagen synthesis.GE and QU also attenuated platelet derived growth factor (PDGF) driven proliferative activity and transforming growth factor ?1 (TGF ?1) driven collagen synthesis.Conclusion:GE and QU can inhibit mouse fibroblast NIH3T3 cell proliferation and collagen synthesis in vitro .
