Topotecan induces caspase-dependent apoptosis in HL-60 cells
- VernacularTitle:金喜素诱导HL-60细胞凋亡依赖caspase活化
- Author:
Xiequn CHEN
;
Youfeng WAN
;
Yunxin CAO
;
- Publication Type:Journal Article
- Keywords:
Topotecan;
HL 60 cells;
apoptosis;
caspase
- From:
Chinese Pharmacological Bulletin
1986;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To gain insight into the biochemical mechanisms of Topotecan induced apoptosis in leukemia cells. METHODS The cytotoxic effect of Topotecan on HL 60 cells was measured by using MTT assay; Topotecan induced apoptosis in HL 60 cells was identified by morphological analysis, DNA agarose gel electropheresis and Annexin V FITC staining; correlation between Topotecan mediated apoptosis and caspase activation was investigated by caspase 8 or caspase 3 inhibitor. RESULTS After incubated with 0 1 ?mol?L -1 TPT for 8,12 and 16 h, survival rate in HL 60 cells significantly reduced to 62%?12%( P
