Protective effects of 9-(4-ethoxycarboxylyphenoxy)-6,7-dimethoxy-1,2,3,4- tetrahydro acridine on anoxia and ischemic injury in cultured PC12 cells
- VernacularTitle:9-(4-乙氧羰基苯氧基)-6,7-二甲氧基-1,2,3,4-四氢吖啶盐酸盐对PC12细胞缺氧缺血损伤的保护作用
- Author:
Rui SHENG
;
Guo-Qing LIU
;
- Publication Type:Journal Article
- Keywords:
Ethoxycarboxylyphenoxy)-6,7-dimethoxy-1,2,3,4-tetrahydro acridine;
PC12 cultured cell;
anoxia injury;
ischemic-like injury
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
1999;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effects of EDT on anoxia and ischemic injury in cultured PC12 cells. Methods Cultured PC12 cells were treated with 1 mmol?L -1 Na 2S 2O 4 and 20 mmol?L -1 NaCN in combination with glucose deprivation. The protective effects of EDT on these two models were evaluated by lactate dehydrogenate (LDH) efflux assay and colormetric MTT assay.ResultsEDT, within the range of 10 -8～ 10 -6 mol?L -1, significantly antagonized LDH efflux induced by two models and increased the optical density at 570 nm tested by colorimetric MTT assay in concentration-dependent manner. 10 -6 mol?L -1 EDT might time-dependently inhibit two injuries and reach maximal level at 48 h. Conclusion EDT can protect PC12 cells from anoxia and ischemic injury.
