Effects of scorpion venom active peptides on the concentration of PGI_2 and NO secreated by human umbilical vein endothelical cells
- VernacularTitle:蝎毒活性多肽对内皮细胞释放PGI_2和NO的影响
- Author:
Yi-Min SONG
;
Xue-Kun LI
;
Xin-Ran LV
;
- Publication Type:Journal Article
- Keywords:
scorpion venom active peptides;
PGI 2;
NO;
enthothelical cells
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
1999;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To further research into the antithrombotic mechanism of scorpion venom active peptides (SVAP). Methods Human umbilical vein endothelial cells (HUVEC) were cultured with enzyme digestive method. After the cultured HUVC was incubated in conditioned media for 1 hour, the effects of SVAP on the concentration of 6-Keto-PGF 1? and NO of HUVEC were determined with radioactive-immunolygic and nitrate reduction enzyme method respectively. Results As compared with control, SVAP in the doses of 1,5,10, 20 mg?L -1 had the distinctive increase of 54%, 68%,72%,79% of the concentration of 6-Keto-PGF 1? and SVAP in the doses of 10, 20 mg?L -1 had the significantly increased of 27%, 46% of the concentration of NO. Regression anylysis showed that the release levels of PGI 2 and NO in HUVEC induced by SVAP was of positive correlation. Conclusion Antithrombotic mechanism of SVAP is related to the increase of PGI 2 and NO released from HUVEC and synergistic and mediating action between NO and PGI 2.
