The rapid regulation of glucocorticoid on high-K~+ induced catecholamine secretion in PC12 cell
- VernacularTitle:糖皮质激素对高钾诱导PC12细胞分泌儿茶酚胺的快速调节作用
- Author:
Shujie LOU
;
Fei CHEN
;
Yizhang CHEN
;
- Publication Type:Journal Article
- Keywords:
glucocorticoids;
catecholamines;
PC12 cells;
potassium
- From:
Academic Journal of Second Military Medical University
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of glucocorticoid on high K + induced catecholamine secretion in PC12 cells. Methods: High performance liquid chromatography with electrochemical detection were used to measure catecholamine secreted by PC12 cells. Results:(1) After PC12 cells were pretreated with various concentration of corticosterone at 37℃ for 5 min and then stimulated with various concentration of high K + for different time periods, the inhibitory effect of corticosterone on catecholamine secretion was found in a dose dependent manner. (2) When the duration of high K + treatment was prolonged, the inhibitory effect of corticosterone on catecholamine secretion tended to be attenuated. Conclusion: Glucocorticoid can rapidly inhibit the catecholamine secretion induced by high K + in PC12 cells. [
