Computer-assisted design of TIMP-1 ribozyme
- VernacularTitle:TIMP-1特异性核酶的计算机设计
- Author:
Jing GUO
;
Dezhong LIU
;
Jianming WU
;
Zihao LIN
;
Youxin JIN
;
- Publication Type:Journal Article
- Keywords:
TIMP 1;
ribonucleases;
computer assisted design;
U 6snRNA
- From:
Academic Journal of Second Military Medical University
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To design the ribozymes to cleave human TIMP 1 mRNA, and embed them into U 6snRNA to make them stable. Methods: Ribozymes were designed according to the “hammerhead structure” described by Symons.Computer was used to analyze the possible cleavage sites. Results: Three ribozymes targeting the nt123, nt299 and nt353 on TIMP 1 mRNA were designed. Embedding ribozyme in U 6snRNA had little effect on its binding with the substrate. Conclusion: Computer assisted design is indispensable in studying ribozyme. Embedding ribozymes in U 6snRNA may be a good way to solve the problems existing in ribozyme study. [
